RAPID SELECTION OF CELL SUBPOPULATION-SPECIFIC HUMAN MONOCLONAL-ANTIBODIES FROM A SYNTHETIC PHAGE ANTIBODY LIBRARY

Peripheral blood leukocytes incubated with a semisynthetic phage antib ody library and fluorochrome-labeled CD3 and CD20 antibodies were used to isolate human single-chain Fv antibodies specific for subsets of b lood leukocytes by flow cytometry. Isolated phage antibodies showed ex clusive binding to the subpopulation used for selection or displayed a dditional binding to a restricted population of other cells in the mix ture. At least two phage antibodies appeared to display hitherto-unkno wn staining patterns of B-lineage cells, This approach provides a subt ractive procedure to rapidly obtain human antibodies against known and novel surface antigens ill their native configuration, expressed on p henotypically defined subpopulations of cells. This approach does not depend on immunization procedures or the necessity to repeatedly const ruct phage antibody libraries.