HUMAN ACETYL-COA CARBOXYLASE - CHARACTERIZATION, MOLECULAR-CLONING, AND EVIDENCE FOR 2 ISOFORMS

We have cloned and sequenced the cDNA coding for human HepG2 acetyl-Co A carboxylase (ACC; EC 6.4.1.2), The sequence has an open reading fram e of 7038 bp that encode 2346 amino acids (M(r), 264,737), The C-termi nal 2.6-kb sequence is very different from that recently reported for human ACC (Ha, J., Daniel, S., Kong, I.-S., Park, C.-K., Tae, H.-J. & Kim, K.-H. [1994] Eur. J. Biochem. 219, 297-306), Northern blot analys is revealed that the ACC mRNA is approximate to 10 kb in size and that its level varies among the tissues tested, Evidence is presented to s how that the human ACC gene is 200-480 kbp in size and maps to chromos ome 17q12, We also provide evidence for the presence of another ACC-li ke gene with similarly sized mRNA but tissue-specific expression diffe rent from that of the ACC gene reported herein. That this second ACC-l ike gene encodes the 280-kDa carboxylase is not ruled out.