DEVELOPMENT OF ENZYME IMMUNOASSAYS FOR THE DETECTION OF PYRETHROID INSECTICIDES .1. MONOCLONAL-ANTIBODIES FOR ALLETHRIN

Three monoclonal antibodies (mab) for the determination of the pyrethr oid insecticide allethrin were generated using a trans-permethric acid -bovine serum albumin conjugate as immunogen. Spleen cells of immunize d BALB/c mice were fused with myeloma cells applying polyethylene glyc ol. Hybridoma cells were selected by HAT selection and screened for ce ll lines producing mab. Three stable cell lines could be isolated for further characterization and development of an indirect competitive en zyme immunoassay. The mab 3/B4-1/F8 showed a preference for S-bioallet hrin, the most efficient insecticidal isomer of allethrin. The detecti on range lay between 1 mu g/L and 250 mu g/L; 50 % inhibition occurred at 46 mu g/L. Cross-reactivities were 43 % for the natural pyrethrins . The mab 1/E2-5/B5 and 1/A5-7/C10 preferred bioallethrin, which is a mixture of two allethrin enantiomers. 50 % inhibition was found at 60 mu g/L or 54 mu g/L, respectively. The tests ranged from 9 mu g/L to 1 70 mu g/L (1/E2-5/B5) and from 6 mu g/L to 160 mu g/L (1/A5-7/C10). Cr oss-reactivities occurred for phenothrin, resmethrin and bioresmethrin .