DETERMINATION OF LACTATE-DEHYDROGENASE BY THE ELECTROCHEMICAL OXIDATION OF NADH AT A MODIFIED MICROBAND GOLD ELECTRODE

Authors
CAI CX JU HX CHEN HY
Citation
Cx. Cai et al., DETERMINATION OF LACTATE-DEHYDROGENASE BY THE ELECTROCHEMICAL OXIDATION OF NADH AT A MODIFIED MICROBAND GOLD ELECTRODE, Analytical letters, 28(5), 1995, pp. 809-820
Citations number
16
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical letters → ACNP
ISSN journal
00032719
Volume
28
Issue
5
Year of publication
1995
Pages
809 - 820
Database
ISI
SICI code
0003-2719(1995)28:5<809:DOLBTE>2.0.ZU;2-T
Abstract
A new amperometric electrode for the assay of lactate dehydrogenase (L DH) is prepared by modifying a nanometer-sized microband gold electrod e with nickel hexacyanoferrate. The amperometric determination bases o n electrochemically detecting NADH produced by the enzymatic reaction of lactate with NAD(+) under the catalysis of LDH. The modified electr ode can sensitively detect LDH at +0.55V vs. SCE with a linear respons e in the range of 10U/mL to 350/mL under optimum conditions.