M. Blanco et al., FIA FLUOROMETRIC-DETERMINATION OF CALCIUM PANTOTHENATE - VALIDATION AND QUANTITATION IN MULTIVITAMIN PREPARATIONS, Analytical letters, 28(5), 1995, pp. 821-833
An FIA method for the determination of calcium pantothenate, one of th
e components of vitamin B complex, is proposed. The method is based on
the alkaline hydrolysis of the analyte to beta-alanine and subsequent
derivatization to a 1-alhylthio-2-alkylisoindole, the fluorescence of
which is monitored at lambda(ex) = 350 nm and lambda(em) = 4.55 nm. C
hemical and physical variables influencing the fluorescence signal wer
e optimized and the method was validated as regards accuracy, repeatab
ility and reproducibility. The proposed method features a broad linear
determination range (0.2-40 mg/L) and a throughput of 25 samples/h. I
t was applied to the determination of calcium pantothenate in multivit
amin preparations with satisfactory results.