ISOLATION OF CDNAS THAT ARE DIFFERENTIALLY EXPRESSED BETWEEN ANDROGEN-DEPENDENT AND ANDROGEN-INDEPENDENT PROSTATE CARCINOMA-CELLS USING DIFFERENTIAL DISPLAY PCR

In the development of prostate cancer there is an important transition from androgen-dependent growth (which can be treated) to androgen-ind ependent growth (which is beyond medical control). This transition is probably accompanied by genetic changes, resulting in the activation o f oncogenes or the inactivation of tumor suppressor genes. In the pres ent manuscript, the isolation of genes that may be involved in advance d, androgen-independent prostate cancer growth is described. Using dif ferential display PCR, 13 cDNAs were isolated representing genes that are differentially expressed between the androgen-dependent prostate c arcinoma cell line LNCaP and the androgen-independent prostate carcino ma cell lines PC-3 and DU 145. These clones were divided into four gro ups: androgen-responsive genes (TL5, TL25, TL32, and TL35); genes with a marked decreased expression in one of the prostate cancer cell line s (TL27); genes with a marked, increased expression in one or more of the prostate cancer cell lines (TL4, TL16, TL21, and TL22); and genes with minor (but repeatable) changes in expression between prostate can cer cell lines (TL7, TL15, TL18, and TL33). The 13 genes were analyzed for their sequence information, tissue specificity, and androgen resp onsiveness in order to identify genes of interest. In summary, differe ntial display PCR appears to provide an attractive alternative to exis ting molecular techniques to screen for differentially expressed genes in prostate cancer cells. (C) 1995 Wiley-Liss, Inc.