DETECTION OF P53 MUTATIONS IN B-CELL NON-HODGKINS-LYMPHOMA CELL-LINES

The p53 tumor suppressor gene is frequently mutated within its evoluti onarily conserved regions in a number of human cancers. Previous repor ts demonstrated mutations of this gene in both Burkitt's lymphoma and B cell chronic lymphocytic leukemia. However, dissimilar results were obtained in non-Hodgkin's lymphoma (NHL). In one study, no mutation wa s detected in 43 NHL tissues. A second study reported p53 mutations in eight (all with advanced stage disease) out of 48 tissues obtained fr om Japanese NHL patients. Using both immunoblotting and radio-immunopr ecipitation, we detected mutant p53 proteins in nine out of 10 B cell lines established from NHL tissues. The mutations were confirmed by re verse transcription polymerase chain reaction-mediated single-strand c onformational polymorphism (RT-PCR-SSCP) analysis in eight cell lines. The high frequency of p53 mutation in NHL B cell lines and the relati vely low frequency of p53 mutations in fresh lymphoma tissue suggests that p53 gene alteration may play a role in lymphomagenesis and/or dis ease progression in a subset of B cell lymphomas and that the p53 muta tion conveys a proliferative advantage on lymphoma cells that permits their in vitro growth.