NUCLEOTIDE-SEQUENCE OF THE BOVINE P53 TUMOR-SUPPRESSOR CDNA

The bovine p53 open reading frame was cloned and characterized using a reverse transcription-polymerase chain reaction amplification (RT-PCR ) method. After sequencing, it appeared that this cDNA is able to code for a 386 amino acids protein with high degree of homology with the o vine p53. Furthermore, the homology is not equally distributed along t he molecule since the highest divergence is located within the exon 4. The bovine p53 shares 93% and 91% homology with the ovine p53 at the DNA and protein level respectively.