VALIDATION OF AN ENZYME-IMMUNOASSAY FOR MEASUREMENT OF EXCRETED ESTROGEN AND TESTOSTERONE METABOLITES IN THE WHITE-CROWNED SPARROW (ZONOTRICHIA LEUCOPHRYS ORIANTHA)
Jv. Lee et al., VALIDATION OF AN ENZYME-IMMUNOASSAY FOR MEASUREMENT OF EXCRETED ESTROGEN AND TESTOSTERONE METABOLITES IN THE WHITE-CROWNED SPARROW (ZONOTRICHIA LEUCOPHRYS ORIANTHA), Zoo biology, 14(2), 1995, pp. 97-106
Over the past few years there has been a growing interest in hormonal
analyses, despite the cost and difficulty involved in measuring hormon
es using radioimmunoassays. Therefore, there is a need for alternate p
rocedures, such as enzyme immunoassays, capable of providing similar d
ata to that provided by radioactive methods. Enzyme immunoassays are a
less expensive method of measuring estrone conjugates and testosteron
e. With further work, the enzyme immunoassay also provides the potenti
al to adapt the enzyme assay for use in the field. The data in this st
udy demonstrate that an enzyme assay can accurately detect both endoge
nous and exogenous hormonal changes in the droppings of white-crowned
sparrows. (C) 1995 Wiley-Liss, Inc.