The effect of iron (Fe) on ureide metabolism was examined in 45-day-ol
d pigeon pea (Cajanus cajan L.) (ureide plant) and alfalfa (Medicago s
ativa) (amide plant). Plants were either inoculated with Rhizobium or
fertilized with ammonium nitrate (NH4NO3). The ureides, allantoin and
allantoate, and allanotinase activity were increased in pigeon pea wit
h Fe supplementation. Specific effect of Fe on ureide metabolism of pi
geon pea was indicated by the lack of same effect in alfalfa under sim
ilar conditions. Nitrogenase activity was elevated with increasing con
centrations of Fe in pigeon pea (ureide) as well as alfalfa (amide) sy
mbiosis. Nitrogen (N2) fixation, ureides, and allantoinase activity we
re reduced at 10 ppm and above concentration of Fe.