DOMINANT LETHAL MUTATIONS, HERITABLE TRANSLOCATIONS, AND UNSCHEDULED DNA-SYNTHESIS INDUCED IN MALE-MOUSE GERM-CELLS BY GLYCIDAMIDE, A METABOLITE OF ACRYLAMIDE

The hypothesis that acrylamide induces dominant lethal mutations and h eritable translocations in male mice, not through direct adduction, bu t by conversion to the reactive epoxide, glycidamide, was investigated . Three studies, namely, induction of dominant lethal mutations, herit able translocations, and unscheduled DNA synthesis in spermatids, whic h were conducted earlier in this laboratory for acrylamide, were also performed for glycidamide to determine its mutagenic properties and to compare responses. Results of these studies are consistent with the p roposal that in vivo conversion to glycidamide is responsible for the mutagenicity of acrylamide in male mice.