Ac. Mork et al., ION-TRANSPORT IN PRIMARY CULTURES FROM HUMAN SWEAT GLAND COILS STUDIED WITH X-RAY-MICROANALYSIS, Cell biology international, 19(2), 1995, pp. 151-159
Chloride secretion in primary cultures of cells originating from om th
e secretory coil of human sweat glands was investigated by electron pr
obe X-ray microanalysis. The total intracellular CI concentration was
lowered by muscarinic agonists (carbachol and acetylcholine), as well
as by the calcium ionophore A23187. The muscarinic agonists also lower
ed the cellular K concentration. Cl- secretion induced by these agonis
ts could be inhibited by the chloride channel blocker NPPB. After cAMP
stimulation, the frequency distribution of the Cl concentration chang
ed from Gaussian to bimodal, indicating that cAMP induces Cl- secretio
n only from a subpopulation of the cells. Also ATP stimulated Cl- secr
etion, indicating the presence of purinergic receptors. The results su
ggest that some of the cells in addition to Ca2+-regulated Cl- channel
s also possess cAMP-activated Cl- channels. Hence, the primary culture
s still possess the Cl- transport mechanisms known to be present in in
tact glands. It can, however, not be excluded that some coil cells hav
e acquired ductal characteristics during culture.