STORAGE OF PLATELET CONCENTRATES FROM OVERNIGHT-STORED BLOOD AND OVERNIGHT-STORED BUFFY COAT - IN-VITRO STUDIES

Platelet concentrates (PCs) were prepared from single buffy coats deri ved from fresh blood and from blood units stored overnight, as well as from buffy coats that were stored overnight. The platelet yield from overnight-stored buffy coats was similar to that of fresh blood or ove rnight-stored blood. PCs were stored at 20-24 degrees C and on day 5 o f storage, platelet aggregation with ADP was tested both at 37 and 25 degrees C. Stored platelets aggregated better at 25 degrees C than 37 degrees C. The maximal aggregation (10 mu M ADP) of stored platelets f rom overnight-stored buffy coats was 46+/-23% (n = 30), while that of stored platelets prepared either from fresh or overnight-stored blood was 27+/-21% (n = 29) and 22+/-15% (n = 29), respectively. Extracellul ar lactate dehydrogenase and ammonia levels, as well as elastase activ ity were similar in stored PCs of different origin. Our conclusion is that PCs prepared from overnight-stored buffy coat might also be suita ble for storage and clinical use. In vivo studies are needed to confir m our findings.