Hr. Barbosa et al., COUNTING OF VIABLE CLUSTER-FORMING AND NON CLUSTER-FORMING BACTERIA -A COMPARISON BETWEEN THE DROP AND THE SPREAD METHODS, Journal of microbiological methods, 22(1), 1995, pp. 39-50
Viable cells from two different bacterial species, namely Escherichia
coli and Beijevinckia derxii were enumerated by three different operat
ors using the Drop and the Spread methods. Statistical analysis showed
that counts obtained by the two methods were significantly different
only for B. derxii, probably due to the influence of the extracellular
slime layer that promotes the formation of cell clusters. Statistical
ly significant differences were also observed when comparing results f
rom the three operators.