SPECIFIC ANTIBODY-MEDIATED DETECTION OF BROCHOTHRIX THERMOSPHACTA IN-SITU IN BRITISH FRESH SAUSAGE

A rabbit polyclonal antibody-linked probe was developed which detected 76% of 800 food isolates of the spoilage bacterium Brochothrix thermo sphacta when cells were bound to nitrocellulose. In slide cross-reacti on tests all six environmental isolates tested were stained but the ty pe strain was not. The antibody did not cross-react with Listeria gray i, L. monocytogenes, Lactobacillus plantarum, Lactococcus lactis, Stre ptococcus mutans, Bacillus cereus or B. subtilis. The antibody-linked probe detected Br. thermosphacta in thin sections of British fresh sau sage when the viable count was greater than 10(6) g(-1). Cells were de tected mainly within 1 or 2 mm of the surface on the loose starchy mat erial. They were not detected within muscle blocks or in the centre of the sausage. Such results suggest that growth of this organism occurs close to the