SALMONELLA-TYPHIMURIUM DT-193 - DIFFERENTIATION OF AN EPIDEMIC PHAGE TYPE BY ANTIBIOGRAM, PLASMID PROFILE, PLASMID FINGERPRINT AND SALMONELLA PLASMID VIRULENCE (SPV) GENE PROBE

Citation
Md. Hampton et al., SALMONELLA-TYPHIMURIUM DT-193 - DIFFERENTIATION OF AN EPIDEMIC PHAGE TYPE BY ANTIBIOGRAM, PLASMID PROFILE, PLASMID FINGERPRINT AND SALMONELLA PLASMID VIRULENCE (SPV) GENE PROBE, Journal of Applied Bacteriology, 78(4), 1995, pp. 402-408
Citations number
25
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
00218847
Volume
78
Issue
4
Year of publication
1995
Pages
402 - 408
Database
ISI
SICI code
0021-8847(1995)78:4<402:SD-DOA>2.0.ZU;2-I
Abstract
Of over 2000 isolates of Salmonella typhimurium DT 193 from humans exa mined in the 2 year period 1991-92, 93% were antibiotic-resistant with the most common R-types being ASSuT (38%) and T (29%). Fourteen plasm id profiles were identified in DT 193 R-type ASSuT with the majority o f isolates being characterized by a single plasmid of 80 MDa (pDEP 34) which in addition to coding for ASSuT, also hybridized with a spy gen e probe prepared from the 50 MDa Salm. dublin serovar-specific plasmid . On the basis of restriction fragment length polymorphisms, two varia nt lines of pDEP 34-like plasmids were identified and a third line whi ch had lost the genes coding for resistance to ampicillin, streptomyci n and sulphonamides, was recognized. Although 18 plasmid profile types were identified in DT 193 R-type T, all isolates carried a high mol. wt plasmid which coded for tetracycline resistance only. Further discr imination was achieved on the basis of hybridization of tetracycline r esistance plasmids with the spy gene probe and restriction enzyme fing erprinting. These results demonstrate that Salm. typhimurium DT 193 ca n be rapidly subdivided by antibiogram and that further subdivision ca n be achieved on the basis of plasmid profile, plasmid fingerprint and hybridization with a spy gene probe.