V. Rani et al., RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FOR GENETIC-ANALYSIS IN MICROPROPAGATED PLANTS OF POPULUS-DELTOIDES MARSH, Plant cell reports, 14(7), 1995, pp. 459-462
RAPD markers were used to assess genetic fidelity of 23 micropropagate
d plants of a single clone (L34) of Populus deltoides. Eleven arbitrar
y 10-base primers were successfully used to amplify DNA from in vivo a
nd in vitro material. Of these, 5 distinguished a total of 13 polymorp
hisms common across 6 micropropagated plants. Apart from these 6 plant
s, the amplification products were monomorphic across all the micropro
pagated plants, the mother plant and 4 additional field-grown control
plants. Our results show that RAPD markers can be used to gain rapid a
nd precise information about genetic similarities or dissimilarities i
n micropropagation systems that might not be so easily evident from ot
her commonly used techniques.