G. Valdimarsson et Gm. Kidder, TEMPORAL CONTROL OF GAP JUNCTION ASSEMBLY IN PREIMPLANTATION MOUSE EMBRYOS, Journal of Cell Science, 108, 1995, pp. 1715-1722
The de novo assembly of gap junctions during compaction in the 8-cell
stage of mouse development is a temporally regulated event. We have pe
rformed experiments designed to explore the relationship between this
event and DNA replication in the second, third, and fourth cell cycles
after fertilization, Inhibition of DNA synthesis by continuous treatm
ent with the DNA synthesis inhibitor, aphidicolin, during the third an
d fourth cell cycles had no effect on the establishment of gap junctio
nal coupling during compaction, However, a delay of 10 hours in DNA sy
nthesis during the second cell cycle caused by a transient aphidicolin
treatment resulted in the failure of gap junctional coupling at the t
ime of compaction, Thus the timing of establishment of gap junctional
coupling, like the timing of compaction itself, is linked to DNA repli
cation in the 2-cell stage, Immunofluorescence analysis showed that th
e failure of gap junctional coupling after aphidicolin treatment in th
e 2-cell stage is correlated with the failure of nascent connexin43 to
be inserted into plasma membranes. We propose that the developmental
'clock' that controls gap junction assembly is set in motion by events
surrounding the second cycle of DNA replication, and that this 'clock
' ultimately controls the post-translational processing of connexin43.