Pg. Parnell et al., IDENTIFICATION OF A MEMBRANE-ASSOCIATED RECEPTOR FOR TRANSFORMING GROWTH-FACTOR TYPE-E, Journal of receptor and signal transduction research, 15(5), 1995, pp. 747-756
We have identified the receptor for epithelial type transforming growt
h factor (TGFe). TGFe, a member of the epithelin/granulin family of pr
oteins, is present primarily in tissues of epithelial origin. It is a
powerful mitogen for epithelial and fibroblastic cells. TGFe, iodinate
d using an immobilized glucose oxidase-lactoperoxidase method, was che
mically crosslinked to receptors on membranes isolated from SW-13 adre
nal carcinoma cells by the crosslinker disuccinimidyl suberate (DSS).
The receptor appears to be a protein which migrates at an apparent mol
ecular weight of approximately 170-175 kDa under reducing and nonreduc
ing conditions in SDS-polyacrylamide gels.