Jx. Mou et al., ATOMIC-FORCE MICROSCOPY OF CHOLERA-TOXIN B-OLIGOMERS BOUND TO BILAYERS OF BIOLOGICALLY RELEVANT LIPIDS, Journal of Molecular Biology, 248(3), 1995, pp. 507-512
Cholera toxin B-oligomer was imaged by atomic force microscopy (AFM) o
n biologically relevant model membranes, such as almitoyI-2-oleoyl-sn-
glycero-3-phosphoethanolamine and egg yolk phosphatidylcholine at room
temperature in solution at a resolution in the range of 1 to 2 nm. In
addition, two-dimensional arrays were grown directly on these model m
embranes without any special treatment, and were also imaged by AFM. T
hese results demonstrate the ability of AFM for imaging membrane prote
ins at high resolution without the need of chemical cross-linking, eit
her within the membrane or to the substratum.