EXPRESSION OF HUMAN GLUTAMATE RECEPTORS (GLUR) IN NEUROBLASTOMA CELL-LINES

Citation
B. Korczak et al., EXPRESSION OF HUMAN GLUTAMATE RECEPTORS (GLUR) IN NEUROBLASTOMA CELL-LINES, NeuroReport, 6(6), 1995, pp. 905-909
Citations number
26
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
09594965
Volume
6
Issue
6
Year of publication
1995
Pages
905 - 909
Database
ISI
SICI code
0959-4965(1995)6:6<905:EOHGR(>2.0.ZU;2-X
Abstract
NEUROBLASTOMA cell lines are useful for the investigation of neuronal receptor regulation since these cells display various neuronal feature s. Here we report the analysis of human AMPA and kainate receptor expr ession in four neuroblastoma cell lines (SK-N-MC, IMR-32, CHP 126 and NMB/N7). The reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated the presence of hGluR1, hGluR4 and EAA4 transcripts in ai l four cell lines whereas hGluR3 mRNA was undetectable. The pattern of expression of hGluR2, EAA1, EAA2, EAA3 and EAA5 was more complex and differed among the cell lines tested. Immunoblot analysis and electrop hysiological recordings failed to demonstrate expression of hGluR1-hGl uR4, EAA1/EAA2 proteins and the formation of functional AMPA/kainate r eceptor channels. These cell lines might provide a valuable model to s tudy the transcriptional and post-transcriptional regulation of AMPA a nd kainate receptor expression.