ANALYSIS OF SELF-INCOMPATIBILITY INTERACTIONS IN 30 RESYNTHESIZED BRASSICA-NAPUS LINES .1. FLUORESCENCE MICROSCOPIC STUDIES

Thirty Brassica napus lines have been developed through interspecific hybridization of B. oleracea and B. campestris lines with defined S-al lele constitutions. These lines, which represent 29 different S-allele combinations, were tested in a diallel of test-pollinations to determ ine the activity of the introgressed S-alleles and intergenomic domina nce relationships. Some consistent trends were observed: B. oleracea S -alleles high in the dominance series (e.g. S-8 S-14, S-29) were alway s active in the resynthesized B. napus lines, whereas recessive S-alle les (S-2, S-15) lost their activity in some test combinations. The B. campestris S-alleles were active in most cases, although 2 alleles wer e partially inactivated by the recessive B. oleracea allele S-15.