ENHANCING PROTEIN SOLUBILIZATION WITH NONDETERGENT SULFOBETAINES

Authors
VUILLARD L MARRET N RABILLOUD T
Citation
L. Vuillard et al., ENHANCING PROTEIN SOLUBILIZATION WITH NONDETERGENT SULFOBETAINES, Electrophoresis, 16(3), 1995, pp. 295-297
Citations number
6
Categorie Soggetti
Biochemical Research Methods
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
16
Issue
3
Year of publication
1995
Pages
295 - 297
Database
ISI
SICI code
0173-0835(1995)16:3<295:EPSWNS>2.0.ZU;2-D
Abstract
A significant increase in the yield of protein extraction by the addit ion of sulfobetaine-type mild solubilization agents is shown for micro somal membrane proteins and proteins from lyophilized platelets, with a maximum increase in yield of up to 100%. These agents are also appli ed to native isoelectric focusing in immobilized pH gradients. The abs ence of significant denaturation induced by these solubilizers is show n for creatine kinase.