This prospective study was conducted among experienced users of period
ic abstinence methods in Sydney, Montreal, and Birmingham (England) in
order to describe the relationship between a laboratory measurement o
f ovulation and the natural symptoms of fertility during breastfeeding
. Daily urinary estrogen and pregnanediol glucuronide assays were used
to estimate the date of ovulation and to determine potentially fertil
e days. A standard set of Symptothermal Method (STM) rules was applied
to daily STM records to assess the correspondence of the natural symp
toms of fertility to the underlying hormonal profile. The STM symptoms
and rules accurately identified 77-94 percent of the women's potentia
lly fertile days, but abstinence was also recommended on about half of
the days when the women were not fertile. An integrated set of common
rules for STM use during breastfeeding is highly sensitive but not sp
ecific in its ability to screen for ovulation.