A. Krichevsky et al., THE DEVELOPMENT OF A PANEL OF MONOCLONAL-ANTIBODIES TO HUMAN LUTEINIZING-HORMONE AND ITS APPLICATION TO IMMUNOLOGICAL MAPPING AND 2-SITE ASSAYS, Endocrine, 2(6), 1994, pp. 511-520
Measurement of human luteinizing hormone in urine may be used to monit
or the ovulatory cycle and is especially useful for large scale epidem
iological studies because of the ease of sample collection. Since urin
ary LH, unlike serum LH, exists in a variety of forms including intact
hormone, subunits and fragments, urinary measurement is complex. It i
s difficult to make immunologic measurements useful for clinical asses
sments especially since it is not known which of the LH forms are most
useful to measure in urine. In order to develop means of measurement
of hLH and its fragments and subunits in urine, we have established an
extensive library of monoclonal antibodies to hLH to enhance the vari
ety of forms of hLH which can be measured in biological fluids. Althou
gh the antibodies were produced by immunization with intact hLH, they
were selected to exhibit sufficient diversity, multiple binding capabi
lities and high affinities to permit such wide-ranging measurements. W
e have also developed an epitope map for hLH using this antibody libra
ry to facilitate construction of two-site antibody measurement systems
. This map indicates the presence of at least seven different epitopes
on the surface of hLH. Three sites are in common with hCG.