D. Inaba et al., THE INFLUENCE OF AIR-DRYING ON HYPER-REMINERALIZATION OF DEMINERALIZED DENTIN - A STUDY ON BULK AS WELL AS ON THIN WET SECTION OF BOVINE DENTIN, Caries research, 29(3), 1995, pp. 231-236
The influence of air-drying on the remineralization of demineralized b
ovine dentine was examined in wet bulk samples, in dried bulk samples
as well as in wet thin sections. Bulk samples of bovine dentine were f
irst demineralized in an acidic gel (pH = 5) at 37 degrees C for 3 wee
ks. After 24-hour pre-treatment of either air-drying or immersion in w
ater, the bulk samples were remineralized in a solution containing 1.5
mM Ca, 0.9 mM phosphate and 10 ppm F- (pH = 7) at 37 degrees C for 2,
4 or 8 days. Separately thin sections prepared from demineralized bul
k dentine were immersed in water for 24 h and were also exposed to the
remineralization solution for 2, 4 or 8 days. The results show that a
ir-drying of the bulk samples increased remineralization of dentine co
nsiderably; the microradiographic parameters (1(d), Delta Z and 1(a))
show that the degree of remineralization ranks: thin wet sections > bu
lk dried > bulk wet. Especially, the remineralization inside lesions w
as greatly enhanced in thin sections and dried samples. It is presumed
that the increased remineralization in dried samples is caused by a '
sponge effect', in which the remineralization fluid is sucked up in a
dried shrunken lesion, resulting in fast and deep penetration of remin
eralization solution and/or presumably increased nucleation.