STEREOSELECTIVE BIOANALYSIS OF OXCARBAZEPINE AND THE ENANTIOMERS OF ITS METABOLITES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The simultaneous liquid chromatographic determination of oxcarbazepine and the enantiomers of its metabolites 10,11-dihydro-10-hydroxycarbam azepine and trans-10,11-dihydroxycarbamazepine in spiked human plasma is described. The compounds are subjected to solid phase extraction be fore chromatography. The separation of the analytes is achieved using chiralcel OD column coupled on line with chiralcel ODH column and a mo bile phase consisting of n-hexane-ethanol (70/30, v/v). The compound w ere detected by ultraviolet absorbance at 220 nm. The limit of quantif ication for each compound was 5 ng/ml.