THE EFFECT OF PLATELET-ACTIVATING-FACTOR ANTAGONIST (BN-52021) ON ACUTE EXPERIMENTAL PANCREATITIS WITH REFERENCE TO MULTIORGAN OXIDATIVE STRESS

Acute hemorrhagic pancreatitis was induced in Wistar rats using a retr ograde intraductal injection of 5% Na-taurocholate. Rats were treated with platelet-activating factor receptor (PAF) antagonist-BN 52021 (5 mg/kg) and sacrificed at 1 and 3 h after induction of acute pancreatit is. Malondialdehyde and sulfhydryl groups concentration were measured in pancreatic, lung, and liver tissue as a parameters of oxidant-antio xidant balance. We have shown that BN 52021 exerts only partial protec ting effect against Na-TC-induced AP in rats. The positive effects of BN 52021 were expressed by: (1) Significant reduction of hyperamylasem ia accompanied by lower malondialdehyde accumulation in pancreatic tis sue; (2) Prevention of sulfl hydryl groups depletion in lung tissue; ( 3) Diminution of necrotic and inflammatory changes in pancreatic tissu e; and (4) Improvement of survival rate. We suggest that these effects may depend on the inhibition of PAF-mediated activation and oxidant g eneration by phagocytes.