Ra. Moran et al., PROBLEMS ASSOCIATED WITH ESTIMATION OF NET CALCIUM-UPTAKE DURING ENAMEL FORMATION USING CA-45, Journal of dental research, 74(2), 1995, pp. 698-701
Ca-45 uptake in mineralizing tissues may occur by net Ca uptake or by
isotopic exchange. It is rarely possible to differentiate between thes
e effects, making interpretation of the findings difficult. Unfortunat
ely, this problem is not often considered, and Ca-45 uptake is usually
regarded as representative of only net calcium uptake. The study repo
rted here was undertaken to estimate the extent to which Ca-45 uptake
in mineralizing enamel is due to net Ca deposition or to isotopic exch
ange, and to consider the implications. The enamel surfaces of the low
er incisors of adult rats were notched at the gingival Line, and the e
ruption distance over 16 hours was measured. This distance was used to
establish the position of a 0.3-mm-wide increment of enamel at the be
ginning and end of the 16-hour period, during which it passed through
the early-maturation stage of enamel formation. The rate of Ca uptake
was determined by chemical assay. Other rats were injected with Ca-45,
mean plasma specific activity values for the experimental period dete
rmined, and the rate of Ca uptake through the same area of enamel form
ation was estimated. The estimates were from two- to nearly ten-fold g
reater than those established by chemical assay, indicating that from
50 to 90% of the Ca-45 uptake occurred by isotopic exchange. Ca-45 upt
ake may indicate more about the labile state of Ca in mineralizing ena
mel than about the rate of mineral deposition.