The removal of humic substances from aqueous solution at pH=4.8 and 6.
0 and 25 degrees C by pure culture of Aspergillus niger has been exami
ned. The removal of humic substances from aqueous phase was monitored
by following the decrease in absorbance at 370 nm. COD and fungal grow
th were also measured. The initial concentration of humic and fulvic a
cids, pH and diameter of fungal pellets were found to be the most impo
rtant parameters. The results suggested that decolorization of batch c
ulture was a consequence of several processes. In the first 5 hours fo
r pellets with diameter 1-3 mm and 10 hours for pellets with diameter
2-5 mm adsorption of humic substances was dominant. Afterwards, desorp
tion of slightly bound humic substances and their degradation, as well
as degradation of remaining humic substances in the solution, continu
ed simultaneously through the period of 10 days.