SOME NEW DEVELOPMENTS IN REDUCTIVE AMINATION WITH COFACTOR REGENERATION

Authors
BOMMARIUS AS DRAUZ K HUMMEL W KULA MR WANDREY C
Citation
As. Bommarius et al., SOME NEW DEVELOPMENTS IN REDUCTIVE AMINATION WITH COFACTOR REGENERATION, Biocatalysis, 10(1-4), 1994, pp. 37-47
Citations number
30
Categorie Soggetti
Biology,"Biothechnology & Applied Migrobiology
Journal title
Biocatalysis → ACNP
ISSN journal
08864454
Volume
10
Issue
1-4
Year of publication
1994
Pages
37 - 47
Database
ISI
SICI code
0886-4454(1994)10:1-4<37:SNDIRA>2.0.ZU;2-U
Abstract
The substrate specificity of LeuDH from Bacillus stearothermophilus an d of PheDH from Rhodococcus sp. was investigated and found to be compl ementary. LeuDH from different organisms have remarkably similar subst rate specificity. During optimization of the synthesis of L-tert-leuci ne, two byproducts (N-alpha-pivaloyl-tert-leucine amide (I) and 2,4-di -tert-butyl-5-hydroxy-imidazol (II)) were found and identified which o riginate from chemical reactions of keto acid and ammonia.