ENZYMATIC-SYNTHESIS OF CARBOHYDRATE-DERIVATIVES USING BETA-GLYCOSIDASE OF SULFOLOBUS-SOLFATARICUS

Enzymatic synthesis of different beta-D-glycosides was obtained using as biocatalyst immobilized cells, crude homogenate, and homogeneous na tive and recombinat beta-glycosidase activity of the thermophilic arch aeon Sulfolobus solfataricus. In particular our investigation was conc erned with the selectivity in the glycosylation of hydroxybenzyl alcoh ols, salicin, 1,2-propanediol, and more complex polyols as well as the use of immobilized cells for the synthesis of hexyl-beta-D-glucoside. The aromatic glucosides obtained by enzyme-catalyzed transglucosylati on were used for kinetic studies of purified Sulfolobus solfataricus e nzyme in the hydrolysis reaction.