INTERACTION BETWEEN THE AUTOKINASE EPSE AND EPSL IN THE CYTOPLASMIC MEMBRANE IS REQUIRED FOR EXTRACELLULAR SECRETION IN VIBRIO-CHOLERAE

Citation
M. Sandkvist et al., INTERACTION BETWEEN THE AUTOKINASE EPSE AND EPSL IN THE CYTOPLASMIC MEMBRANE IS REQUIRED FOR EXTRACELLULAR SECRETION IN VIBRIO-CHOLERAE, EMBO journal, 14(8), 1995, pp. 1664-1673
Citations number
71
Categorie Soggetti
Biology
Journal title
ISSN journal
02614189
Volume
14
Issue
8
Year of publication
1995
Pages
1664 - 1673
Database
ISI
SICI code
0261-4189(1995)14:8<1664:IBTAEA>2.0.ZU;2-U
Abstract
Vibrio cholerae secretes a number of proteins important for virulence, including cholera toxin. This process requires the products of the ep s genes which have homologues in genera such as Aeromonas, Klebsiella and Pseudomonas and are thought to form a membrane-associated multipro tein complex. Here we show that the putative nucleotide-binding protei n EpsE is associated with and stabilized by the cytoplasmic membrane v ia interaction with EpsL. Analysis of fusion proteins between EpsE and the homologous ExeE from Aeromonas hydrophila demonstrates that the N -terminus of EpsE contains the EpsL binding domain and determines spec ies specificity. An intact Walker A box, commonly found in ATP-binding proteins, is required for activity of EpsE in vivo and for autophosph orylation of purified EpsE in vitro. These results indicate that both the kinase activity of EpsE as well as its ability to interact with th e putative cytoplasmic membrane protein EpsL are required for transloc ation of toxin across the outer membrane in Vibrio cholerae.