NEUROMEDIN-C BINDS CU(II) AND NI(II) VIA THE ATCUN MOTIF - IMPLICATIONS FOR THE CNS AND CANCER GROWTH

Neuromedin C is a bombesin-like neuropeptide of the sequence Gly-Asn-H is-Trp-Ala-Val-Gly-His-Leu-Met-NH2. Characterization of the amino term inal Cu(II), NL(II) binding motif in albumins led us to predict that a ny other peptides or proteins with the same motif would also bind Cu(I I) and Ni(II) specifically (1). The primary sequence of neuromedin C c ontains the motif in the form Gly-Asn-His. Neuromedin C was therefore predicted to bind Cu(II) and Ni(II) specifically. The studies presente d here confirm that prediction. These findings may have implications f or the transport of Cu(II) within the central nervous system as well a s both Menkes disease and Wilson disease. Both are genetic copper meta bolism disorders which are characterized by severe neurological sympto ms. In addition, Cu(II) may interfere with the neurotransmission or gr owth factor effects of neuromedin C. (C) 1995 Academic Press, Inc.