QUANTITATIVE-ANALYSIS OF NONSTEROIDAL ANTIINFLAMMATORY DRUGS BY CAPILLARY ZONE ELECTROPHORESIS

The potential utility of capillary zone electrophoresis (CZE) for the separation and quantitative determination of some non-steroidal anti-i nflammatory drugs (NSAIDs) was investigated. The influence of differen t parameters on migration times, peak symmetry, efficiency and resolut ion was studied; these parameters included the nature and concentratio n of the anionic and cationic components of the separation buffer. A b uffer consisting of 75 mM glycine adjusted to FH 9.1 with triethanolam ine was found to provide a very efficient and stable electrophoretic s ystem for the CZE analysis of NSAIDs, giving RSD values of about 0.1 a nd 0.5% for the within-day reproducibility of migration times and peak areas, respectively at a concentration of 25 mu g ml(-1) (n = 5). Res ponse was linear from 2-100 mu g ml(-1) for both sulindac and tiaprofe nic acid, for which the LOQ values were 2.8 and 1.9 mu g ml(-1), respe ctively, using UV detection at 280 nm. Accuracy for each drug was 102- 103%.