USE OF PRINCIPAL COMPONENT ANALYSIS FOR THE EVALUATION OF THE RETENTION BEHAVIOR OF MONOAMINE-OXIDASE INHIBITORY DRUGS ON BETA-CYCLODEXTRINCOLUMN

The retention of 17 monoamine oxidase inhibitory drugs (proparlgylamin e derivatives) were determined on a beta-cyclodextrin polymer (beta CD P)-coated silica column using ethanol-0.05 M K2HPO4 (6:4 v/v) as the e luent. The relative strength of interaction between the drugs and a wa ter soluble beta-cycodextrin polymer was determined by charge-transfer chromatography carried out on reversed-phase TLC layers. The relation ship between capacity factors, physicochemical parameters and inclusio n complex forming capacity of the monoamine oxidase inhibitory drugs w ere evaluated by stepwise regrerssion analysis and by principal compon ent analysis (PCA) followed by two-dimensional nonlinear mapping and v arimax rotation. Calculations indicated that the retention of monoamin e oxidase inhibitory drugs on beta CDP column is mainly governed by th eir steric and lipophylic parameters. Significant linear correlations were found between the corresponding coordinates of varimax rotation a nd two-dimensional nonlinear maps proving the suitability of both meth ods for the reduction of dimensionality of complicated data matrices.