M. Cancino et al., GENERATION AND CHARACTERIZATION OF 3 MONOCLONAL-ANTIBODIES USEFUL IN DETECTING AND DISTINGUISHING BEAN GOLDEN MOSAIC-VIRUS ISOLATES, Phytopathology, 85(4), 1995, pp. 484-490
Two monoclonal antibodies prepared to purified virions of bean golden
mosaic virus (BGMV) isolates from Guatemala (GA) and the Dominican Rep
ublic (DR) and one monoclonal antibody prepared to the coat protein of
a BGMV isolate from Brazil (BZ) expressed in Escherichia coli were us
eful serological probes for detection of whitefly-transmitted geminivi
ruses. Abutilon mosaic, BGMV isolates from Puerto Rico (PR), and Homes
tead, Florida (H), BGMV-BZ, -DR, and -GA isolates, euphorbia mosaic, r
hynchosia mosaic, squash leaf curl, soybean yellow mosaic, an isolate
from Macroporous lathyroides in Florida, and tomato mottle geminivirus
es all reacted efficiently in Western blot and enzyme-linked immunosor
bent assay with monoclonal antibody 3F7, indicating that this monoclon
al antibody has a broad spectrum of reactivity. Another monoclonal ant
ibody (2G5) reacted only with BGMV-DR, -GA, and -PR isolates. A third
monoclonal antibody (5C5) reacted only with BGMV-BZ. A new geminivirus
infecting cabbage (Brassicaceae) in Florida did not react with any of
the three monoclonal antibodies.