THE MODEL ELECTROPHORETIC SPECTRA OF HMW GLIADINS AND GLUTENINS OF COMMON WHEAT-VARIETIES CULTIVATED IN THE CZECH-REPUBLIC IN 1993

The work is a catalogue of model electrophoretic spectra of HMW gliadi ns and glutenins of all wheat varieties, approved for cultivation in 1 993. The catalogue serves for fast and objective determination of vari etal trueness and purity of doses of seeds and merchanting of wheat; 2 1 varieties of winter wheat and five varieties of spring wheat, repres ented by the seed of grade S1, were assessed in total. To determine el ectrophoretic composition of gliadins (60 grains) vertical electrophor esis in columns of starch gel Al-lactate buffer at pH 3.1 with 2 mol.l (-1) of urea (Sasek, Sykorova, 1989) was used. Allelic gliadin blocks were separated from eiectrophoretic spectra of gliadins after Sobko, P operelja (1986). To determine electrophoretic spectra of HMW glutenins the procedure of vertical discontinual electrophoresis in PAA gel und er presence of SDS (Laemmli, 1970) was applied. Allelic blocks of zone s or allelic zones of HMW glutenins were determined after Payne et al. (1981). The degree of protein polymorphism was assessed and genetic s tructure of varieties assessed has been determined; 22 varieties out o f 26 assessed varieties are homogenous in the structure of gliadins, h ence they are pure lines. The other four varieties are populations com posed of two sister lines. In the composition of HMW glutenins only tw o varieties are heterogenous. Different varieties or their protein lin es were assessed according to the occurrence of allelic blocks of glia dins and glutenins of HMW - markers of baking quality and frost-hardin ess.