EVALUATION OF PROTEIN AND DNA POLYMORPHISM IN LENTIL (LENS-CULINARIS L) FOR GENOTYPES AND CULTIVARS DISTINGUISHING

Several analytical methods were used for lentil cultivars and lines di stinguishing. Seed protein patterns obtained after SDS-PAGE were very similar and not usable for these purposes. The same results were obtai ned by RFLP analyses when rDNA unit of barley was used as a probe. Of 118 genotypes tested by esterase profiles, 14 different EST banding pa tterns were obtained; 1I bands of esterase activity with different mob ility were detected in the gels. Each of genotypes had two to seven ba nds. RAPD polymorphism between tested cultivars was detected. The comb ination of several biochemical and molecular-biological methods and cl assical agronomicai-morphological descriptors could be helpful for len til genotypes identification.