S. Farooq et al., IDENTIFICATION OF DIFFERENT WHEAT GENOTYPES THROUGH POLYMORPHISM BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD), Pakistan journal of botany, 26(2), 1994, pp. 373-382
Polymerase chain reaction was used to detect polymorphism in 10 differ
ent salt-tolerant wheat introgression lines produced through wide hybr
idization, 2 cultivated hexaploid wheat varieties, a tetraploid wheat
variety and salt-tolerant accession D of Aegilops cylindrica. DNAs ext
racted from fresh leaves by the CTAB method were amplified using 25 mu
L reaction volume in a Perkin Elmer Thermal Cycler and randomly sequen
ced 10 mer synthetic primers. Based on amplification reactions, all th
e primers were divided into 4 categories. Category A primers reacted w
ith all the wheat lines, category B with none, category C with 10-14,
and category D with 3-7 wheat lines. Of the 18 reactive primers, 11(61
%) detected polymorphism in all the test material. The level of polymo
rphism was low and ranged from 1.2 to 22.8%. Most of the primers produ
ced monomorphic bands including very intense, easily visible and less
visible bands. Of the 9 salt-tolerant introgression wheat lines, 6 wer
e indentified on the basis of polymorphic bands. Our study indicated p
ossibilities for using Random Amplified Polymorphic DNA (RAPD) markers
to detect specific variations in the genome that could be used for va
rietal finger printing.