IDENTIFICATION OF DIFFERENT WHEAT GENOTYPES THROUGH POLYMORPHISM BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

Polymerase chain reaction was used to detect polymorphism in 10 differ ent salt-tolerant wheat introgression lines produced through wide hybr idization, 2 cultivated hexaploid wheat varieties, a tetraploid wheat variety and salt-tolerant accession D of Aegilops cylindrica. DNAs ext racted from fresh leaves by the CTAB method were amplified using 25 mu L reaction volume in a Perkin Elmer Thermal Cycler and randomly sequen ced 10 mer synthetic primers. Based on amplification reactions, all th e primers were divided into 4 categories. Category A primers reacted w ith all the wheat lines, category B with none, category C with 10-14, and category D with 3-7 wheat lines. Of the 18 reactive primers, 11(61 %) detected polymorphism in all the test material. The level of polymo rphism was low and ranged from 1.2 to 22.8%. Most of the primers produ ced monomorphic bands including very intense, easily visible and less visible bands. Of the 9 salt-tolerant introgression wheat lines, 6 wer e indentified on the basis of polymorphic bands. Our study indicated p ossibilities for using Random Amplified Polymorphic DNA (RAPD) markers to detect specific variations in the genome that could be used for va rietal finger printing.