INTERNAL SEQUENCE-ANALYSIS OF PROTEINS ELUTED FROM POLYACRYLAMIDE GELS

We have developed an elution-digestion-sequencing (EDS) method, which yields the internal amino acid sequence of partially purified proteins . The overall yield for the method was greater than 60%. The method yi elded peptide peaks that could be sequenced on HPLC for all tested pro teins with masses from 45 to 200 . 10(3) and yielded internal amino ac id sequence information when as little as 10 pmol of partially purifie d protein was used as the starting material. The EDS method was extrem ely reliable and gave sequence information for each of 25 proteins tes ted, including high-molecular-mass proteins (M(r)>100 . 10(3)) that we re difficult to sequence by other methods.