Gene frequencies for the human platelet antigen systems HPA-1, -2, -3,
and -5 were determined directly from DNA isolated from cord blood of
more than 900 randomly selected Caucasoid newborns in Vienna, Austria.
Genotyping was performed by specific amplification of the respective
regions coding for platelet glycoproteins GP Ib, IIb, IIIa, and Ia by
PCR. These PCR products were analyzed after restriction enzyme digesti
on and electrophoresis. The observed gene frequencies were: HPA-1a: 0.
852, HPA-1b: 0.148; HPA-2a: 0.918, HPA-2b: 0.082; HPA-3a: 0.612, HPA-3
b: 0.388; HPA-5a: 0.892, HPA-5b: 0.108. There was a good fit with the
Hardy-Weinberg equilibrium. Results from serological determinations an
d genotyping showed no discrepancies.