POTENT ANTIVIRAL ACTIVITY OF AN ANTISENSE OLIGONUCLEOTIDE COMPLEMENTARY TO THE INTRON-EXON BOUNDARY OF HUMAN CYTOMEGALOVIRUS GENES UL36 ANDUL37

Authors
PARI GS FIELD AK SMITH JA
Citation
Gs. Pari et al., POTENT ANTIVIRAL ACTIVITY OF AN ANTISENSE OLIGONUCLEOTIDE COMPLEMENTARY TO THE INTRON-EXON BOUNDARY OF HUMAN CYTOMEGALOVIRUS GENES UL36 ANDUL37, Antimicrobial agents and chemotherapy, 39(5), 1995, pp. 1157-1161
Citations number
26
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
39
Issue
5
Year of publication
1995
Pages
1157 - 1161
Database
ISI
SICI code
0066-4804(1995)39:5<1157:PAAOAA>2.0.ZU;2-3
Abstract
An antisense phosphorothioate oligonucleotide complementary to the int ron-exon boundary of human cytomegalovirus genes UL36 and UL37 (UL36AN TI) reduced the yield of infectious virus by 99% and inhibited human c ytomegalovirus DNA replication at a concentration of 0.08 mu M. In add ition, oligonucleotides with base substitutions which resulted in base pair mismatches showed lesser degrees of activity, indicating a seque nce-specific antisense mechanism. UL36ANTI was also shown to inhibit D NA replication of ganciclovir-resistant strains and human cytomegalovi rus clinical isolates.