Rf. Frye et Dd. Stiff, DETERMINATION OF CHLORZOXAZONE AND 6-HYDROXYCHLORZOXAZONE IN HUMAN PLASMA AND URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 686(2), 1996, pp. 291-296
Citations number
13
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
A sensitive and reproducible method is described for the determination
of the cytochrome P450 enzyme 2E1 substrate chlorzoxazone and its pri
mary metabolite 6-hydroxychlorzoxazone in human plasma and urine. Plas
ma or diluted urine were acidified, incubated with beta-glucuronidase
and then were extracted with diethyl ether. Separation of the analytes
was achieved on a C-18 column with UV detection set at 283 nm. Excell
ent linearity was observed over the concentration ranges of 100-3000 n
g/ml and 4-400 mu g/ml in plasma and urine, respectively. The intra-as
say variability was less than or equal to 5.1% and the inter-assay var
iability was less than or equal to 8.2% for each compound in each matr
ix. The method presented is applicable to pharmacokinetic and pharmaco
genetic studies utilizing chlorzoxazone.