PURIFICATION AND CHARACTERIZATION OF A FACTOR WHICH INHIBITS RETROVIRUS REPLICATION

Authors
KRIEF P LAWRENCE D ALILECHE A PLAISANCE S JASMIN C SEMMEL M
Citation
P. Krief et al., PURIFICATION AND CHARACTERIZATION OF A FACTOR WHICH INHIBITS RETROVIRUS REPLICATION, Research in virology, 146(2), 1995, pp. 113-123
Citations number
25
Categorie Soggetti
Virology
Journal title
Research in virology → ACNP
ISSN journal
09232516
Volume
146
Issue
2
Year of publication
1995
Pages
113 - 123
Database
ISI
SICI code
0923-2516(1995)146:2<113:PACOAF>2.0.ZU;2-6
Abstract
Chick embryo cells (CEC) secrete a factor which inhibits HIV1 replicat ion. Non-productive infection of CEC with vesicular stomatitis virus i ncreases 5-10-fold the synthesis of this factor, After purification by FPLC the biological activity is associated with a heat-resistant prot ein of approximately 150 kDa, composed of two subunits of 70 and 58 kD a and charged negatively. Monoclonal antibodies raised against this pr otein block the inhibitory activity of the purified protein and react with the 150-kDa and 58-kDa proteins in Western blots.