ADVANTAGE OF A RAPID EXTRACTION METHOD OF HIV-1 DNA SUITABLE FOR POLYMERASE CHAIN-REACTION

We describe a new protocol for extraction of DNA suitable for HIV1 gen e amplification from clinical samples using ''Chelex-100'' chelating r esin. Comparison was made with the classic proteinase K extraction met hod; 154 specimens were extracted with both methods and subjected to P CR (polymerase chain reaction). The Chelex-100 procedure optimized the yield of DNA recovery and minimized contamination due to sample manip ulation. It decreased false negative results due to PCR inhibitors. A DNA sample suitable for use in PCR was obtained in 30 minutes. Chelex- 100 treatment is a simple, rapid and low-cost method for DNA extractio n in clinical laboratories.