C. Vignoli et al., ADVANTAGE OF A RAPID EXTRACTION METHOD OF HIV-1 DNA SUITABLE FOR POLYMERASE CHAIN-REACTION, Research in virology, 146(2), 1995, pp. 159-162
We describe a new protocol for extraction of DNA suitable for HIV1 gen
e amplification from clinical samples using ''Chelex-100'' chelating r
esin. Comparison was made with the classic proteinase K extraction met
hod; 154 specimens were extracted with both methods and subjected to P
CR (polymerase chain reaction). The Chelex-100 procedure optimized the
yield of DNA recovery and minimized contamination due to sample manip
ulation. It decreased false negative results due to PCR inhibitors. A
DNA sample suitable for use in PCR was obtained in 30 minutes. Chelex-
100 treatment is a simple, rapid and low-cost method for DNA extractio
n in clinical laboratories.