DEPENDENCE OF THE GENE-EXPRESSION LEVEL I N ESCHERICHIA-COLI FROM THETRANSLATION INITIATION REGION (TIR) STRUCTURE .1. THE PRIMARY STRUCTURE OF TIR

Authors
GUREVICH AI ESIPOV RS KACHALINA TA KAYUSHIN AL KOROSTELEVA MD
Citation
Ai. Gurevich et al., DEPENDENCE OF THE GENE-EXPRESSION LEVEL I N ESCHERICHIA-COLI FROM THETRANSLATION INITIATION REGION (TIR) STRUCTURE .1. THE PRIMARY STRUCTURE OF TIR, Bioorganiceskaa himia, 21(2), 1995, pp. 117-123
Citations number
34
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
21
Issue
2
Year of publication
1995
Pages
117 - 123
Database
ISI
SICI code
0132-3423(1995)21:2<117:DOTGLI>2.0.ZU;2-I
Abstract
The comparison of expression levels of two genes - interleukin-3 (il3) and epidermal growth factor connected to lider peptide of OmpF (lompe gf)- was carried out using specially constructed plasmids contained va rious structures of translational enhancers. It was shown that besides already known binding sires from mRNA translation initiation region ( TIR) to 16S rRNA (SD, UB1 and DB), there is additional binding site of TIR disposed from -30 to -60 nt upsteam of start codon AUG (UB2) havi ng significant increasing effect on translation initiation and corresp ondingly on expression level.