DO-STAT FED-BATCH PRODUCTION OF CIS,CIS-MUCONIC ACID FROM BENZOIC-ACID BY PSEUDOMONAS-PUTIDA BM014

For the production of cis,cis-muconic acid via biocatalytic conversion reactions from a toxic cosubstrate, benzoic acid, we developed a fed- batch process using computer-controlled DO-stat feeding. The mutant st rain of Pseudomonas putida BM014 produced cis,cis-muconic acid with hi gh conversion yield. More than 32 g/l of cis,cis-muconic acid was accu mulated in 40 h including the initial batch and constant-feeding perio ds and a productivity value more than 2.2 g/(l . h) was achieved.