CYTOCHROME B(562) - ELECTROACTIVE PROTEIN FOR BIOELECTRONICS

Authors
SASABE H FURUNO T KOBAYASHI K KOBAYASHI Y NAGAMUNE T
Citation
H. Sasabe et al., CYTOCHROME B(562) - ELECTROACTIVE PROTEIN FOR BIOELECTRONICS, Molecular crystals and liquid crystals science and technology. Section A, Molecular crystals and liquid crystals, 255, 1994, pp. 271-278
Citations number
10
Categorie Soggetti
Crystallography
Journal title
Molecular crystals and liquid crystals science and technology. Section A, Molecular crystals and liquid crystals → ACNP
ISSN journal
1058725X
Volume
255
Year of publication
1994
Pages
271 - 278
Database
ISI
SICI code
1058-725X(1994)255:<271:CB-EPF>2.0.ZU;2-3
Abstract
To develop bioelectronic devices and/or the structural analyses of pro teins, two-dimensional crystallization of proteins is required. Heme p roteins are particularly interesting due to electron transfer characte ristics associated with the porphyrin-based prosthetic group. Cytochro me b(562) has been chosen as a model protein for the fabrication of tw o-dimensional crystals. Three approaches in making two-dimensional cry stals of cytochrome b(562) are presented here: (1) the formation of mo nomolecular layer of cytochrome b(562) reconstituted with alkylated he me, (2) the control of surface charge density of cytochrome b(562) and (3) the enhancement of thermal stability both by means of protein eng ineering.