DIFFERENTIAL REGULATION OF A GLIAL FIBRILLARY ACIDIC PROTEIN-LACZ TRANSGENE IN RETINAL ASTROCYTES AND MULLER CELLS

Purpose. Glial fibrillary acidic protein (GFAP) is normally expressed in astrocytes, but not in Muller cells, in the mouse retina. In respon se to retinal injury or photoreceptor degeneration, however, GFAP gene transcription is strongly activated in the Muller cell. To identify t he genetic elements involved in GFAP gene induction, the authors have studied gene expression in transgenic mice in which beta-galactosidase (beta-gal) expression is under control of GFAP regulatory sequences. Methods. Histochemical methods were used to study gene expression. The transgene expression was followed by x-gal staining, whereas GFAP exp ression was monitored by immunostaining with GFAP antibody. Results. I n GFAP-LacZ transgenic mice, beta-gal activity and GFAP immunostaining were found in retinal astrocytes. Transgene expression showed the sam e developmental pattern as that of endogenous GFAP in retinal astrocyt es. In addition, beta-gal staining also was observed in lens epithelia l cells. Neither GFAP nor beta-gal expression was seen in Muller cells in the adult or developing retina. When focal retinal lesions were in troduced into the retina, strong GFAP immunostaining was observed in M uller cells throughout the retina. No beta-gal staining was seen in Mu ller cells in these retinas. In astrocytes, however, beta-gal and GFAP both were present. Conclusions. The observations suggest that cis ele ments responsible for GFAP expression in retinal astrocytes are presen t in 5' flanking region of the GFAP gene, whereas the regulatory eleme nts involved in GFAP induction in Muller cells are located elsewhere.